Gene Expression Calculator

🧬 Expression Data Input

Gene Name / ID

Reference Gene (Housekeeping)

Control Expression Level

Treatment Expression Level

Control Std Dev

Treatment Std Dev

Control Sample Size (n)

Treatment Sample Size (n)

Read Count

Gene Length (bp)

Total Mapped Reads (millions)

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Enter expression data and click Calculate to see results

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Expression comparison chart will appear here after calculation

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Previous calculations will appear here

ΔCt = Ct(target) - Ct(reference) ΔΔCt = ΔCt(treatment) - ΔCt(control) Fold Change = 2^(-ΔΔCt)

Upregulated Fold change > 1.5

Downregulated Fold change < 0.67

Significant p-value < 0.05

Highly Significant p-value < 0.01

RPKM = (Read Count × 10^9) / (Gene Length × Total Mapped Reads)